{"id":149,"date":"2022-03-22T17:38:40","date_gmt":"2022-03-22T17:38:40","guid":{"rendered":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/?p=149"},"modified":"2022-03-22T18:22:06","modified_gmt":"2022-03-22T18:22:06","slug":"library-of-influenza-h1-pseudotypes-with-an-hiv-backbone","status":"publish","type":"post","link":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2022\/03\/22\/library-of-influenza-h1-pseudotypes-with-an-hiv-backbone\/","title":{"rendered":"Library of Influenza H1 pseudotypes with an HIV backbone"},"content":{"rendered":"<p><strong>Strain (accession, expression plasmid, protease for HA cleavage)<\/strong><\/p>\n<p>A\/South Carolina\/1\/1918 (AF117241.1 phCMV1 TMPRSS4)<\/p>\n<p>A\/Puerto Rico\/8\/1934 (AF389118.1 pI.18 TMPRSS4)<\/p>\n<p>A\/New Caledonia\/20\/1999 (EU103824.1 phCMV1 TMPRSS4)<\/p>\n<p>A\/duck\/Italy\/1447\/2005 (HF563054.1 pI.18 TMPRSS4)<\/p>\n<p>A\/Solomon Islands\/3\/2006 (EU124177.1 pI.18 TMPRSS4)<\/p>\n<p>A\/Brisbane\/59\/2007 (CY163864.1 pI.18 TMPRSS4)<\/p>\n<p>A\/California\/7\/2009 (CY121680.1 pI.18 TMPRSS4)<\/p>\n<p>A\/Texas\/05\/2009 (GQ457487.1 pI.18 HAT)<\/p>\n<p>A\/England\/195\/2009 (GQ166661.1 pEVAC TMPRSS4)<\/p>\n<p>A\/Bolivia\/559\/2013 (EPI466837 pEVAC TMPRSS4)<\/p>\n<p>A\/swine\/Guangxi\/1\/2013_12_26_4 (KJ725056 pEVAC TMPRSS4)<\/p>\n<p>A\/Michigan\/45\/2015 (EPI662594 pEVAC TMPRSS4)<\/p>\n<p>A\/Slovenia\/2903\/2015 (EPI768541 pEVAC TMPRSS4)<\/p>\n<p>A\/Brisbane\/02\/2018 (EPI1383389 pEVAC TMPRSS4)<\/p>\n<p>A\/swine\/Henan\/SN10\/2018_02__4 (MN416619 pEVAC TMPRSS4)<\/p>\n<p>A\/swine\/Beijing\/0301\/2018_03__4 (MN416589 pEVAC TMPRSS4)<\/p>\n<p>&nbsp;<\/p>\n<p><img src=\"https:\/\/www.mdpi.com\/vaccines\/vaccines-09-00741\/article_deploy\/html\/images\/vaccines-09-00741-g002.png\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>Schematic representation of the production of influenza H1 pseudotypes by plasmid transfection. (a) Using a 4 plasmid system, exogenous NA was added to the transfected cultures at least 8 h post-transfection. (b) Addition of protease, which is necessary for the production of H1 pseudotypes is optimized to increase production titres by transfecting using a \u2018checkerboard\u2019 approach with different proteases (e.g., HAT, <strong>TMPRSS4<\/strong>, and TMPRSS2). Protease plasmid was added at a ratio of 1:1, 1:0.5, and 1:0.25 to HA plasmid DNA for rapid optimization in a 6 well plate format. All pseudotypes were harvested after 48 h in culture. ORCID: <a href=\"https:\/\/orcid.org\/0000-0002-7978-3815\">https:\/\/orcid.org\/0000-0002-7978-3815<\/a><\/p>\n<p>&nbsp;<\/p>\n<ul class=\"kent-social-links\"><li><a href='http:\/\/www.facebook.com\/sharer.php?u=https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2022\/03\/22\/library-of-influenza-h1-pseudotypes-with-an-hiv-backbone\/&amp;t=Library of Influenza H1 pseudotypes with an HIV backbone' target='_blank'><i class='ksocial-facebook' title='Share via Facebook'><\/i><\/a><\/li><li><a href='http:\/\/twitter.com\/home?status=Library of Influenza H1 pseudotypes with an HIV backbone%20https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2022\/03\/22\/library-of-influenza-h1-pseudotypes-with-an-hiv-backbone\/' target='_blank'><i class='ksocial-twitter' title='Share via Twitter'><\/i><\/a><\/li><li><a href='https:\/\/plus.google.com\/share?url=https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2022\/03\/22\/library-of-influenza-h1-pseudotypes-with-an-hiv-backbone\/' target='_blank'><i class='ksocial-google-plus' title='Share via Google Plus'><\/i><\/a><\/li><li><a href='http:\/\/linkedin.com\/shareArticle?mini=true&amp;url=https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2022\/03\/22\/library-of-influenza-h1-pseudotypes-with-an-hiv-backbone\/&amp;title=Library of Influenza H1 pseudotypes with an HIV backbone' target='_blank'><i class='ksocial-linkedin' title='Share via Linked In'><\/i><\/a><\/li><li><a href='mailto:content=https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2022\/03\/22\/library-of-influenza-h1-pseudotypes-with-an-hiv-backbone\/&amp;title=Library of Influenza H1 pseudotypes with an HIV backbone' target='_blank'><i class='ksocial-email' title='Share via Email'><\/i><\/a><\/li><\/ul>","protected":false},"excerpt":{"rendered":"<p>Strain (accession, expression plasmid, protease for HA cleavage) A\/South Carolina\/1\/1918 (AF117241.1 phCMV1 TMPRSS4) A\/Puerto Rico\/8\/1934 (AF389118.1 pI.18 TMPRSS4) A\/New Caledonia\/20\/1999 (EU103824.1 phCMV1 TMPRSS4) A\/duck\/Italy\/1447\/2005 (HF563054.1 &hellip; <a href=\"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2022\/03\/22\/library-of-influenza-h1-pseudotypes-with-an-hiv-backbone\/\">Read&nbsp;more<\/a><\/p>\n","protected":false},"author":2395,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[194094,1],"tags":[],"_links":{"self":[{"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/posts\/149"}],"collection":[{"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/users\/2395"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/comments?post=149"}],"version-history":[{"count":3,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/posts\/149\/revisions"}],"predecessor-version":[{"id":158,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/posts\/149\/revisions\/158"}],"wp:attachment":[{"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/media?parent=149"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/categories?post=149"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/tags?post=149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}