{"id":134,"date":"2020-11-09T13:13:25","date_gmt":"2020-11-09T13:13:25","guid":{"rendered":"http:\/\/blogs.kent.ac.uk\/mspvpuprepare\/?p=134"},"modified":"2021-05-22T18:45:30","modified_gmt":"2021-05-22T17:45:30","slug":"library-of-influenza-ha-pseudotypes-h1-h16-for-measuring-ha-directed-antibody-responses","status":"publish","type":"post","link":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2020\/11\/09\/library-of-influenza-ha-pseudotypes-h1-h16-for-measuring-ha-directed-antibody-responses\/","title":{"rendered":"Library of Influenza A and B HA pseudotypes (H1-H18, Yam\/Vic\/Pre) for measuring HA directed antibody responses"},"content":{"rendered":"<p>Influenza pseudotypes have been employed extensively by us to undertake serological assays for public\/animal health and vaccine industry R&amp;D, and could potentially contribute towards the licensure of new influenza vaccines, at least as an adjunct assay. In a <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0264410X09011049?via%3Dihub\">joint study<\/a> with Novartis Vaccines, we showed that pseudotypes could be used to sensitively quantify neutralising antibody responses elicited by a pre-pandemic H5N1 vaccine and that these correlated significantly with those measured by haemagglutination inhibition (HI), single radial haemolysis (SRH) and microneutralisation (MN). We have made an <a href=\"https:\/\/www.mdpi.com\/2409-9279\/1\/1\/8\">optimised protocol<\/a> available for undertaking such pseudotype neutralisation assays.<\/p>\n<p>We now have a comprehensive library of influenza A HA pseudotypes comprising HA1 to HA18 and influenza B pseudotypes comprising Yam\/Vic and the pre-lineage split. This is an excellent resource for undertaking immunogenicity studies for universal influenza vaccines.<\/p>\n<p>We have published our library as a pre-print <a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2021.05.20.444964v1\">here<\/a>.<\/p>\n<p>Please get in touch at n.temperton@kent.ac.uk to discuss access and collaboration.<\/p>\n<ul class=\"kent-social-links\"><li><a href='http:\/\/www.facebook.com\/sharer.php?u=https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2020\/11\/09\/library-of-influenza-ha-pseudotypes-h1-h16-for-measuring-ha-directed-antibody-responses\/&amp;t=Library of Influenza A and B HA pseudotypes (H1-H18, Yam\/Vic\/Pre) for measuring HA directed antibody responses' target='_blank'><i class='ksocial-facebook' title='Share via Facebook'><\/i><\/a><\/li><li><a href='http:\/\/twitter.com\/home?status=Library of Influenza A and B HA pseudotypes (H1-H18, Yam\/Vic\/Pre) for measuring HA directed antibody responses%20https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2020\/11\/09\/library-of-influenza-ha-pseudotypes-h1-h16-for-measuring-ha-directed-antibody-responses\/' target='_blank'><i class='ksocial-twitter' title='Share via Twitter'><\/i><\/a><\/li><li><a href='https:\/\/plus.google.com\/share?url=https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2020\/11\/09\/library-of-influenza-ha-pseudotypes-h1-h16-for-measuring-ha-directed-antibody-responses\/' target='_blank'><i class='ksocial-google-plus' title='Share via Google Plus'><\/i><\/a><\/li><li><a href='http:\/\/linkedin.com\/shareArticle?mini=true&amp;url=https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2020\/11\/09\/library-of-influenza-ha-pseudotypes-h1-h16-for-measuring-ha-directed-antibody-responses\/&amp;title=Library of Influenza A and B HA pseudotypes (H1-H18, Yam\/Vic\/Pre) for measuring HA directed antibody responses' target='_blank'><i class='ksocial-linkedin' title='Share via Linked In'><\/i><\/a><\/li><li><a href='mailto:content=https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2020\/11\/09\/library-of-influenza-ha-pseudotypes-h1-h16-for-measuring-ha-directed-antibody-responses\/&amp;title=Library of Influenza A and B HA pseudotypes (H1-H18, Yam\/Vic\/Pre) for measuring HA directed antibody responses' target='_blank'><i class='ksocial-email' title='Share via Email'><\/i><\/a><\/li><\/ul>","protected":false},"excerpt":{"rendered":"<p>Influenza pseudotypes have been employed extensively by us to undertake serological assays for public\/animal health and vaccine industry R&amp;D, and could potentially contribute towards the &hellip; <a href=\"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/2020\/11\/09\/library-of-influenza-ha-pseudotypes-h1-h16-for-measuring-ha-directed-antibody-responses\/\">Read&nbsp;more<\/a><\/p>\n","protected":false},"author":2395,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[194094],"tags":[],"_links":{"self":[{"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/posts\/134"}],"collection":[{"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/users\/2395"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/comments?post=134"}],"version-history":[{"count":5,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/posts\/134\/revisions"}],"predecessor-version":[{"id":144,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/posts\/134\/revisions\/144"}],"wp:attachment":[{"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/media?parent=134"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/categories?post=134"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/mspvpuprepare\/wp-json\/wp\/v2\/tags?post=134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}