{"id":1470,"date":"2016-11-02T09:48:37","date_gmt":"2016-11-02T09:48:37","guid":{"rendered":"http:\/\/blogs.kent.ac.uk\/biosciences\/?p=1470"},"modified":"2016-11-02T09:48:37","modified_gmt":"2016-11-02T09:48:37","slug":"research-seminar-the-molecular-mechanisms-of-epigenetic-regulatory-repression-complexes","status":"publish","type":"post","link":"https:\/\/blogs.kent.ac.uk\/biosciences\/2016\/11\/02\/research-seminar-the-molecular-mechanisms-of-epigenetic-regulatory-repression-complexes\/","title":{"rendered":"Research Seminar: The molecular mechanisms of epigenetic regulatory repression complexes."},"content":{"rendered":"<p><b>Professor John <\/b><b>Schwabe,<\/b> <strong>Department Molecular and Cell Biology, University of Leicester<\/strong><\/p>\n<p><b>Tuesday 8th November, 1.00 p.m., Stacey Lecture Theatre 1<\/b><\/p>\n<p>&nbsp;<\/p>\n<p>My group is seeking to understand the molecular mechanisms that\u00a0underlie the epigentic reprogramming of the genome during\u00a0cellular\u00a0differentiation and development. At least five large, multiprotein Histone\u00a0Deacetylase (HDAC) complexes are key players.\u00a0They trigger the programmed\u00a0repression of regions of the genome which is one of the critical first steps.\u00a0Interestingly these\u00a0complexes also play a role in the expression of active\u00a0genes.<\/p>\n<p>We have led the field in using crystallographic studies to explore the\u00a0assembly of the SMRT:HDAC3:TBL1:GPS2 complex, the\u00a0NuRD complex HDAC1:MTA1:RBBP4\u00a0and the MiDAC complex. These studies led to the unexpected and breakthrough\u00a0discovery\u00a0that HDAC complexes are regulated by small molecule inositol\u00a0phosphates.<\/p>\n<p>More\u00a0recently we\u00a0have taken an integrated approach combining crystal structures with\u00a0cross-linking\/mass spectrometry, SAXS\u00a0and negative stain EM to build a more complete picture of these complexes.<\/p>\n<p>We\u00a0are now working towards cryo-EM structures for the NuRD (750 kDa), MIDAC\u00a0(450kDa), CoREST (200kDa), Sin3A (260kDa)\u00a0and SMRT (420kDa) complexes.<\/p>\n<p>&nbsp;<\/p>\n<ul class=\"kent-social-links\"><li><a href='http:\/\/www.facebook.com\/sharer.php?u=https:\/\/blogs.kent.ac.uk\/biosciences\/2016\/11\/02\/research-seminar-the-molecular-mechanisms-of-epigenetic-regulatory-repression-complexes\/&amp;t=Research Seminar: The molecular mechanisms of epigenetic regulatory repression complexes.' target='_blank'><i class='ksocial-facebook' title='Share via Facebook'><\/i><\/a><\/li><li><a href='http:\/\/twitter.com\/home?status=Research Seminar: The molecular mechanisms of epigenetic regulatory repression complexes.%20https:\/\/blogs.kent.ac.uk\/biosciences\/2016\/11\/02\/research-seminar-the-molecular-mechanisms-of-epigenetic-regulatory-repression-complexes\/' target='_blank'><i class='ksocial-twitter' title='Share via Twitter'><\/i><\/a><\/li><li><a href='https:\/\/plus.google.com\/share?url=https:\/\/blogs.kent.ac.uk\/biosciences\/2016\/11\/02\/research-seminar-the-molecular-mechanisms-of-epigenetic-regulatory-repression-complexes\/' target='_blank'><i class='ksocial-google-plus' title='Share via Google Plus'><\/i><\/a><\/li><li><a href='http:\/\/linkedin.com\/shareArticle?mini=true&amp;url=https:\/\/blogs.kent.ac.uk\/biosciences\/2016\/11\/02\/research-seminar-the-molecular-mechanisms-of-epigenetic-regulatory-repression-complexes\/&amp;title=Research Seminar: The molecular mechanisms of epigenetic regulatory repression complexes.' target='_blank'><i class='ksocial-linkedin' title='Share via Linked In'><\/i><\/a><\/li><li><a href='mailto:content=https:\/\/blogs.kent.ac.uk\/biosciences\/2016\/11\/02\/research-seminar-the-molecular-mechanisms-of-epigenetic-regulatory-repression-complexes\/&amp;title=Research Seminar: The molecular mechanisms of epigenetic regulatory repression complexes.' target='_blank'><i class='ksocial-email' title='Share via Email'><\/i><\/a><\/li><\/ul>","protected":false},"excerpt":{"rendered":"<p>Professor John Schwabe, Department Molecular and Cell Biology, University of Leicester Tuesday 8th November, 1.00 p.m., Stacey Lecture Theatre 1 &nbsp; My group is seeking &hellip; <a href=\"https:\/\/blogs.kent.ac.uk\/biosciences\/2016\/11\/02\/research-seminar-the-molecular-mechanisms-of-epigenetic-regulatory-repression-complexes\/\">Read&nbsp;more<\/a><\/p>\n","protected":false},"author":32117,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[50580],"tags":[],"_links":{"self":[{"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/posts\/1470"}],"collection":[{"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/users\/32117"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/comments?post=1470"}],"version-history":[{"count":1,"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/posts\/1470\/revisions"}],"predecessor-version":[{"id":1471,"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/posts\/1470\/revisions\/1471"}],"wp:attachment":[{"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/media?parent=1470"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/categories?post=1470"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.kent.ac.uk\/biosciences\/wp-json\/wp\/v2\/tags?post=1470"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}