{"id":1255,"date":"2016-03-09T11:44:37","date_gmt":"2016-03-09T11:44:37","guid":{"rendered":"http:\/\/blogs.kent.ac.uk\/biosciences\/?p=1255"},"modified":"2016-03-09T11:44:37","modified_gmt":"2016-03-09T11:44:37","slug":"research-seminar-applying-single-molecule-sequencing-to-diagnostics-and-microbiomes","status":"publish","type":"post","link":"https:\/\/blogs.kent.ac.uk\/biosciences\/2016\/03\/09\/research-seminar-applying-single-molecule-sequencing-to-diagnostics-and-microbiomes\/","title":{"rendered":"Research Seminar: Applying single molecule sequencing to diagnostics and microbiomes."},"content":{"rendered":"

Dr. Justin Pachebat, Institute of Biological, Environmental and Rural Sciences, Aberystwyth University<\/strong><\/p>\n

Tuesday 15th March, 1.00 p.m., Cornwallis Octagon Lecture Theatre 2 (COLT2)<\/strong><\/p>\n

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Genome sequencing been revolutionized by the advent of next generation sequencing (NGS) platforms. Whereby once it would take years to sequence a single organism, researchers can now sequence multiple genomes within a week. This has lead to a paradigm shift in pathogen research, the study of microbiomes and clinical diagnostics. Genome sequencing can give a wealth of information about infectivity, phylogeny, and host specificity, but there are still significant challenges in the generation of genome sequence data from samples in a diagnostic setting.<\/p>\n

This talk will discuss the advantages and disadvantages of using: 1) single molecule genomics and NGS technologies to characterise pathogens of animals, humans and plants; 2) meta-genomic and meta-transcriptomic approaches to study host \u2013 pathogen – microbiome interactions. Examples covered will include the development of techniques to sequence Cryptosporidium from clinical isolates; detection of novel human enteric viruses; the use of meta-genomics to characterize the lung microbiome in COPD and lung cancer patients.<\/p>\n

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Biography:<\/strong><\/p>\n

Started off as a parasitologist, then discovered single molecule genomics and next generation sequencing. After a PhD on malaria vaccine targets at the MRC NIMR, I spent 7 years as a post-doc in the Single Molecule Genomics laboratory at the MRC LMB, before moving to the University of Cambridge where I developed an interest in next generation sequencing. Since 2010 I have been a Senior Lecturer in microbial genomics at IBERS, Aberystwyth University.<\/p>\n

My research has two main focuses: 1) using single molecule genomics and NGS technologies to characterise pathogens of animals, humans and plants with the aim of developing novel diagnostics \/ methods of control; 2) using meta-genomic and meta-transcriptomic approaches to study host \u2013 pathogen – microbiome interactions.<\/p>\n

Current examples of my research include:<\/p>\n