Research Seminar: Extracting information from imaging data by fitting mathematical models

Professor Nigel Burroughs, Mathematics Institute and Warwick Systems Biology Centre, University of Warwick

Tuesday 16th October, 1.00 p.m., Stacey Lecture Theatre 1

With the increasing sophistication of microscope technology (e.g. super resolution, light sheet), interpreting the data and extracting reliable information increasingly requires mathematical and statistical methods. I will discuss work with Andrew McAinsh on imaging kinetochore dynamics and conformational changes. During metaphase, congressed chromosome oscillate across the metaphase plate. By fitting a mechanical model to those oscillations we inferred the forces acting on the kinetochore which lead to the tension-clock model of directional switching. This lead to an investigation of kinetochore compliance to forces and distinguishing conformational changes under loading and microtubule attachment.