Dr. Renier van der Hoorn, Head of Plant Chemetics Lab, University of Oxford
Monday 20th January 2014, 4.00 p.m., Marlowe Lecture Theatre 1
Activity-based protein profiling (ABPP) is a powerful functional proteomics approach to display the active proteome using small molecule probes that covalently and irreversibly react with the active site residue of proteins. Labeled proteins can be displayed on protein gels and purified and identified by mass spectrometry. This approach displays the availability and reactivity of active site residues of proteins, which is a hallmark for protein activities. I will give examples of specific probes, broad-range probes and probes that have unexpected labeling sites that are still informative on the activity of proteins. Using examples from plant science, I will show that ABPP can be broadly applied on different organisms, both on extracted proteomes and on living cells or whole organisms.